Deleted
Sample Discontinued
Experience: None
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Coding

Part:BBa_K2668000:Design

Designed by: Younes Bouchiba   Group: iGEM18_Toulouse-INSA-UPS   (2018-08-15)


Cerberus tetrameric (E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 376
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 324


Design Notes

To be purified, this part was cloned in double digested pET28 (NcoI / HindIII) for being expressed with a His Tag only if the unnatural amino acid incorporation occured.


Source

The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible . The streptavidin part comes from Streptomyces avidinii.

References